Chlorophyll fluorescence quenching in isolated light harvesting complexes induced by zeaxanthin

FEBS Letters

Wentworth, Mark¹  Horton, Peter¹  Ruban, Alexander V.¹ 

[1] Robert Hill Institute for Photosynthesis Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK

关键词: Non-photochemical quenching;    Light harvesting complex;    Xanthophyll cycle;    Chlorophyll fluorescence;    Thylakoid membrane;    DBMIB;    2;    5-dibromo-3-methyl-6-isopropyl-ρ-benzoquinone;    DM;    n-dodecyl β-maltoside;    Lhcb;    proteins encoded by the Lhcb genes;    LHC II;    the light harvesting complexes of photosystem II;    NPQ;    non-photochemical quenching;    PS II;    photosystem II;    qE;    non-photochemical quenching dependent upon the ΔpH;    ΔpH;    transthylakoid pH gradient;   

DOI  :  10.1016/S0014-5793(00)01369-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Non-photochemical quenching of chlorophyll fluorescence in plants occurs in the light harvesting antenna of photosystem II and is regulated by the xanthophyll cycle. A new in vitro model for this process has been developed. Purified light harvesting complexes above the detergent critical micelle concentration have a stable high fluorescence yield but a rapidly inducible fluorescence quenching occurs upon addition of zeaxanthin. Violaxanthin was without effect, lutein and antheraxanthin induced a marginal response, whereas the violaxanthin analogue, auroxanthin, induced strong quenching. Quenching was not caused by aggregation of the complexes but was accompanied by a spectral broadening and red shift, indicating a zeaxanthin-dependent alteration in the chlorophyll environment.

【 授权许可】

Unknown