Light‐induced stimulation of carbonic anhydrase activity in pea thylakoids

FEBS Letters

Ignatova, Lyudmila K¹  Kollmeier, Michelle A¹  Ivanov, Boris N¹  Moskvin, Oleg V¹ 

[1]Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia

关键词: Thylakoid membrane;    Proton transfer;    Energy conversion;    Bicarbonate;    CO2 concentration;   

DOI  :  10.1016/S0014-5793(00)01328-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Stimulation of the bicarbonate dehydration reaction in thylakoid suspension under conditions of saturating light at pH 7.6–8.0 was discovered. This effect was inhibited by nigericin or the lipophilic carbonic anhydrase (CA) inhibitor ethoxyzolamide (EZ), but not by the hydrophilic CA inhibitor, acetazolamide. It was shown that the action of EZ is not caused by an uncoupling effect. It was concluded that thylakoid CA is the enzyme utilizing the light-generated proton gradient across the thylakoid membrane thus facilitating the production of CO₂ from HCO₃ ⁻ and that this enzyme is covered from the stroma side of thylakoids by a lipid barrier.

【 授权许可】

Unknown