The survival motor neuron protein interacts with the transactivator FUSE binding protein from human fetal brain

FEBS Letters

Williams, Barbara Y¹  Hamilton, Susan L¹  Sarkar, Hemanta K¹ 

[1] Department of Molecular Physiology and Biophysics, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA

关键词: Spinal muscular atrophy;    Survival motor neuron;    FUSE binding protein;    Yeast two-hybrid;   

DOI  :  10.1016/S0014-5793(00)01320-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To identify interacting proteins of survival motor neuron (SMN) in neurons, a fetal human brain cDNA library was screened using the yeast two-hybrid system. One identified group of SMN interacting clones encoded the DNA transactivator FUSE binding protein (FBP). FBP overexpressed in HEK293 cells or endogenously expressed in fetal and adult mouse brain bound specifically in vitro to recombinant SMN protein. Furthermore, an anti-FBP antibody specifically co-immunoprecipitated SMN when both proteins were overexpressed in HEK293 cells. These results demonstrate that FBP is a novel interacting partner of SMN and suggests a possible role for SMN in neuronal gene expression.

【 授权许可】

Unknown