Phenotype‐dependent expression of cadherin 6B in vascular and visceral smooth muscle cells

FEBS Letters

Matsuzawa, Yuji²  Miyata, Shoji¹  Hayashi, Ken'ichiro³  Sobue, Kenji³  Nishida, Wataru³  Kimura, Kazuhiro³  Chimori, Yoshihiro³  Shimane, Miyuki¹  Funahashi, Shin-ichi¹ 

[1] Chugai Research Institute for Molecular Medicine, Inc., 153-2 Nagai, Niihari, Ibaraki 300-4101, Japan;Department of Internal Medicine and Molecular Science, Osaka University Graduate School of Medicine (B5), 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan;Division of Neurochemistry and Neuropharmacology, Department of Neuroscience, Biomedical Research Center, Osaka University Graduate School of Medicine (C13), 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan

关键词: mRNA subtraction;    Smooth muscle;    Phenotypic modulation;    Cadherin 6B;   

DOI  :  10.1016/S0014-5793(00)01220-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We used mRNA subtraction of differentiated and dedifferentiated smooth muscle cells (SMCs) to reveal the molecular mechanisms underlying the phenotypic modulation of SMCs. With this approach, we found that a 10 kb mRNA encoding a homotypic cell adhesion molecule, cadherin 6B, was strongly expressed in differentiated vascular and visceral SMCs, but not in the dedifferentiated SMCs derived from them. In vivo, cadherin 6B was expressed in vascular and visceral SMCs, in addition to brain, spinal cord, retina and kidney, at a late stage of chicken embryonic development. These results suggest that cadherin 6B is a novel molecular marker for vascular and visceral SMC phenotypes and is involved in the late differentiation of SMCs.

【 授权许可】

Unknown