Expression of type XVI collagen in cultured skin fibroblasts is related to cell growth arrest

FEBS Letters

Akagi, Atsushi²  Tanaka, Nobuhiko²  Ishibashi, Akira²  Tajima, Shingo²  Yamaguchi, Noriko¹  Kawada, Akira³ 

[1] Medical Research Institute, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan;Department of Dermatology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan;Kinki University School of Medicine, 377-2 Ohnohigashi, Sayama City, Osaka 589-8511, Japan

关键词: Type XVI collagen;    Cell adhesion;    Type I and III collagen;    Skin fibroblast;   

DOI  :  10.1016/S0014-5793(00)01241-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The expression of type XVI collagen in various phases of cell growth in cultured skin fibroblasts was studied. A marked increase in type XVI collagen mRNA level was found in stationary phases of cell growth (non-adherent and confluent phases), whereas the expression of type I and III collagens was undetectable in the non-adherent phase but became greater in the confluent phase. When suspended cells were further cultured over 72 h (suspension arrest), mRNA level and gene transcription of type XVI collagen were time-dependently increased whereas those of type I collagen remained undetectable. When the confluent cells were further cultured for 72 h under the condition of serum deprivation (serum deprivation arrest), mRNA levels of both type XVI collagen and type I collagen were elevated. The level of type XVI collagen polypeptide in the culture media of suspension-arrested and serum deprivation-arrested cells paralleled the mRNA level of type XVI collagen. The results indicate that expression of type XVI collagen (a member of the fibril-associated collagens with interrupted triple helices), unlike interstitial collagens (type I collagen), is related to cell growth arrest brought about by two different growth inhibiting systems, suspension arrest and serum deprivation arrest.

【 授权许可】

Unknown