FEBS Letters | |
Two different modes of cyclin Clb2 proteolysis during mitosis in Saccharomyces cerevisiae | |
Braus, Gerhard H1  Irniger, Stefan1  Bäumer, Matthias1  | |
[1] Institute of Microbiology and Genetics, Georg-August-University, Grisebachstrasse 8, D-37077 Göttingen, Germany | |
关键词: Anaphase-promoting complex; Cyclin degradation; Cdh1; Cdc15; Mitotic exit; Ubiquitin-dependent proteolysis; APC/C; anaphase-promoting complex/cyclosome; CDK; cyclin-dependent kinase; OD; optical density; | |
DOI : 10.1016/S0014-5793(00)01208-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Sister chromatid separation and mitotic exit are triggered by the anaphase-promoting complex (APC/C) which is a multi-subunit ubiquitin ligase required for proteolytic degradation of various target proteins. Cdc20 and Cdh1 are substrate-specific activators of the APC/C. It was previously proposed that Cdh1 is essential for proteolysis of the yeast mitotic cyclin Clb2. We show that Clb2 proteolysis is triggered by two different modes during mitosis. A fraction of Clb2 is degraded during anaphase in the absence of Cdh1. However, a second fraction of Clb2 remains stable during anaphase and is degraded in a Cdh1-dependent manner as cells exit from mitosis. Most of cyclin Clb3 is degraded independently of Cdh1. Our data imply that degradation of mitotic cyclins is initiated by a Cdh1-independent mechanism.
【 授权许可】
Unknown
【 预 览 】
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