The polyomavirus major capsid protein VP1 interacts with the nuclear matrix regulatory protein YY1

FEBS Letters

Forstová, Jitka¹  Palková, Zdena²  Španielová, Hana¹  Hollanderová, Dana¹  Amati, Paolo²  Gottifredi, Vanesa² 

[1] Department of Genetics and Microbiology, Faculty of Natural Sciences, Charles University, Vinièná 5, 12844 Prague 2, Czech Republic;Instituto Pasteur - Fondazione Cenci Bolognetti, Dipartimento di Biotecnologie Cellulari ed Ematologia, Sezione di Genetica Molecolare, Università di Roma La Sapienza, Viale Regina Elena 324, 00161 Rome, Italy

关键词: Polyomavirus;    VP1;    Nuclear matrix;    Yin Yang 1;   

DOI  :  10.1016/S0014-5793(00)01170-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polyomavirus reaches the nucleus in a still encapsidated form, and the viral genome is readily found in association with the nuclear matrix. This association is thought to be essential for viral replication. In order to identify the protein(s) involved in the virus-nuclear matrix interaction, we focused on the possible roles exerted by the multifunctional cellular nuclear matrix protein Yin Yang 1 (YY1) and by the viral major capsid protein VP1. In the present work we report on the in vivo association between YY1 and VP1. Using the yeast two-hybrid system we demonstrate that the VP1 and YY1 proteins physically interact through the D-E region of VP1 and the activation domain of YY1.

【 授权许可】

Unknown