Phospholipase D2: functional interaction with caveolin in low‐density membrane microdomains

FEBS Letters

Lavie, Yaakov¹  Liscovitch, Mordechai¹  Czarny, Malgorzata¹  Fiucci, Giusy¹  Banno, Yoshiko²  Nozawa, Yoshinori² 

[1] Department of Biological Regulation, Weizmann Institute of Science, Rehovot 76100, Israel;Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500, Japan

关键词: Phospholipase D;    Caveolin;    Lipid raft;    Caveolae;    Sterol regulatory element-binding protein;    HaCaT keratinocyte;    ALLN;    acetyl-leucyl-leucyl-norleucinal;    C6-NBD;    [6-N-(7-nitrobenzo-2-oxa-1;    3 diazol-4-yl)amino]caproyl;    EGF-R;    epidermal growth factor receptor;    PC;    phosphatidylcholine;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    PLD;    phospholipase D;    SREBP;    sterol regulatory element-binding protein;   

DOI  :  10.1016/S0014-5793(00)01174-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Low-density detergent-insoluble membrane domains contain caveolin-1 and are enriched in a phospholipase D activity that is not PLD1. Here we show that caveolin-rich fractions, prepared from HaCaT human keratinocytes by either detergent-based or detergent-free methods, contain PLD2. Caveolar membrane PLD activity is stimulated 2-fold by low concentrations (10–30 μM) of the caveolin-1 and caveolin-2 scaffolding domain peptides, whereas it is inhibited at higher concentrations of the peptides. Immunoisolated HA-tagged PLD1 and PLD2 are not stimulated by the peptides, although both enzymes retain sensitivity to their inhibitory effect. Down-regulation of caveolin-1 expression by treatment of the cells with acetyl-leucyl-leucyl-norleucinal decreased caveolar PLD activity by 50%. Similarly, expression of an active form of the sterol regulatory element-binding protein (SREBP_1–490) down-regulated caveolin-1 expression by 50% and decreased caveolar PLD activity by 60%. These data identify the PLD activity in caveolin-rich membranes as PLD2 and provide in vivo evidence suggesting that caveolin-1 regulates PLD2 activity.

【 授权许可】

Unknown