The Saccharomyces cerevisiae DNA damage checkpoint is required for efficient repair of double strand breaks by non‐homologous end joining

FEBS Letters

de la Torre-Ruiz, Maria-Angeles¹  Lowndes, Noel F.¹ 

[1] Imperial Cancer Research Fund, Clare Hall Laboratories, CDC Laboratory, South Mimms, Hertfordshire EN6 3LD, UK

关键词: DNA damage;    Checkpoint;    Repair;    Non-homologous end joining;    Ku;   

DOI  :  10.1016/S0014-5793(00)01180-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this work we report that the Saccharomyces cerevisiae RAD9, RAD24, RAD17, MEC1, MEC3 and RAD53 checkpoint genes are required for efficient non-homologous end joining (NHEJ). RAD9 and RAD24 function additionally in this process. Defective NHEJ in rad9Δ–rad24Δ, but not yku80Δ cells, is only partially rescued by imposing G1 or G2/M delays. Thus, checkpoint functions other than transient cell cycle delays may be required for normal levels of NHEJ. Epistasis analysis also indicated that YKU80 and RAD9/RAD24 function in the same pathway for repair of lesions caused by MMS and γ-irradiation. Unlike NHEJ, the checkpoint pathway is not required for efficient site-specific integration of plasmid DNA into the yeast genome, which is RAD52-dependent, but RAD51-independent.

【 授权许可】

Unknown