Suppression of GD1α ganglioside‐mediated tumor metastasis by liposomalized WHW‐peptide

FEBS Letters

Ogino, Koichi¹  Taki, Takao¹  Takikawa, Masako²  Asai, Tomohiro²  Tanaka, Michinori¹  Ishikawa, Dai¹  Yamaguchi, Naoto²  Kikkawa, Hironori²  Oku, Naoto² 

[1] Molecular Medical Science Institute, Otsuka Pharmaceutical Co., Ltd., Kagasuno 463-10, Kawauchi, Tokushima 771-0192, Japan;Department of Radiobiochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Yada 52-1, Shizuoka 422-8526, Japan

关键词: Cell adhesion;    Experimental liver metastasis;    Liposome;    Phage-displayed random peptide library;    GD1α-replica peptide;   

DOI  :  10.1016/S0014-5793(00)01110-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

GD1α ganglioside-replica peptides were recently isolated from a phage-displayed random pentadecapeptide library by assaying for inhibition of adhesion of RAW117-H10 lymphosarcoma cells to hepatic sinusoidal microvessel endothelial (HSE) cells. We show here that the Trp-His-Trp (WHW) peptide was identified as a minimal sequence of the GD1α-replica peptide WHWRHRIPLQLAAGR. The addition of WHW peptide-attached liposomes displayed efficient inhibition of liver metastasis of RAW117-H10 cells as well as of GD1α-mediated adhesion of RAW117-H10 cells to HSE cells in vitro. These results suggest that engineered liposomes for peptide delivery are applicable to treatment for metastasis.

【 授权许可】

Unknown