Isolation and expression of a novel alternatively spliced mu opioid receptor isoform, MOR‐1F

FEBS Letters

Pasternak, Gavril W.¹  Pan, Ying-Xian¹  Chang, Albert¹  Mahurter, LoriAnn¹  Xu, Jin¹  Bolan, Elizabeth¹  Rossi, Grace¹ 

[1] The Cotzas Laboratory of Neuro-Oncology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA

关键词: Morphine;    Morphine-6β-glucuronide;    Analgesia;    Splicing;    MOR-1;    Antisense mapping;   

DOI  :  10.1016/S0014-5793(00)01095-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The MOR-1 gene is large, with a recent study reporting nine exons spanning 250 kb which combine to yield six different mu opioid receptor splice variants. We now report the isolation of exon 10, which is contained within yet another splice variant, MOR-1F, which is composed of exons 1, 2, 3, 10, 6, 7, 8 and 9. Exon 10 comprises 186 bp which predict a unique 58 amino acid sequence extending beyond exon 3. It has been mapped between exons 4 and 6 and has flanking consensus splice sequences. On Northern blot analysis, the MOR-1F mRNA is smaller than the other MOR-1 mRNAs. When expressed in CHO cells, MOR-1F binds the mu opioid radioligand [³H]DAMGO with high affinity (K _D=1.04±0.03 nM). Competition studies demonstrated the selectivity of the variant for mu opioid ligands, supporting its classification within the mu opioid receptor family.

【 授权许可】

Unknown