Requirements for alternative forms of the activator domain, P5abc, in the Tetrahymena ribozyme

FEBS Letters

Naito, Yoshikazu¹  Shiraishi, Hideaki¹  Inoue, Tan¹ 

[1]Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan

关键词: Ribozyme;    P5abc;    Tertiary interaction;    Tetrahymena;   

DOI  :  10.1016/S0014-5793(99)01797-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of P5abc domain of the Tetrahymena LSU self-splicing Group I intron is to enhance the activity of the intron via tertiary interactions involving A-rich bulge and terminal loops L5b and L5c. We constructed and examined alternative forms of the domain that accelerate the ribozymatic reaction. The results indicate that the characteristic structure of P5c subdomain plays an important role by forming L2×L5c interaction (P14) and that the region flanking P5c subdomain can be significantly mutable without much affecting the activity of the ribozyme.

【 授权许可】

Unknown