Gene structure of human cholecystokinin (CCK) type‐A receptor: body fat content is related to CCK type‐A receptor gene promoter polymorphism

FEBS Letters

Kono, Akira¹  Takiguchi, Sohichi¹  Funakoshi, Akihiro⁵  Yamamori, Shunji⁴  Takata, Yutaka¹  Matsumoto, Hideo⁴  Shimokata, Hiroshi³  Kataoka, Kazuhiro¹  Matsusue, Kimihiko¹  Miyasaka, Kyoko² 

[1]Department of Chemotherapy, National Kyushu Cancer Center, Fukuoka 811-1395, Japan

[2]Department of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan

[3]Department of Epidemiology, National Institute for Longevity Sciences, Ohbu 474-8522, Japan

[4]Department of Cellular Genetics, Mitsubishi Kagaku Bio-Clinical Laboratories, INC, Tokyo 174-8555, Japan

[5]Department of Gastroenterology, National Kyushu Cancer Center, 3-1-1 Notame, Minami-ku, Fukuoka 811-1395, Japan

关键词: Fat deposition;    CCK-A receptor promoter;    Capsite Hunting;    Polymorphism;    Leptin;   

DOI  :  10.1016/S0014-5793(00)01080-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The transcriptional start site of the human cholecystokinin (CCK)-A receptor gene was determined by the Capsite Hunting method. Two sequence changes were detected, a G to T change in nucleotide −128, and an A to G change in nucleotide −81. The homozygote (T/T, G/G) was detected in 25 of 1296 individuals (1.9%) in the cohort study. This polymorphism showed a significantly higher percent body fat and higher levels of serum insulin and leptin, compared with wild type and heterozygotes. Our study provided the possibility that polymorphism in the promoter region of the CCK-A receptor gene may be one of genetic factors affecting fat deposition.

【 授权许可】

Unknown