Induction of tcI 7, a gene encoding a β‐subunit of proteasome, in tobacco plants treated with elicitins, salicylic acid or hydrogen peroxide 1

FEBS Letters

Suty, Lydie¹  Blein, Jean-Pierre¹  Etienne, Philippe¹  Petitot, Anne-Sophie¹  Houot, Valérie¹ 

[1] Unité Associée INRA-Université de Bourgogne, Phytopharmacie et Biochimie des Interactions Cellulaires, INRA BV 1540, 21034 Dijon Cedex, France

关键词: Elicitin;    Hydrogen peroxide;    Salicylic acid;    Proteasome β-subunit;    Systemic acquired resistance;    Tobacco;    GSP;    gene-specific primer;    GST;    glutathione S-transferase;    HR;    hypersensitive reaction;    nPCR;    nested polymerase chain reaction;    PAL;    phenylalanine ammonia-lyase;    PCR;    polymerase chain reaction;    Ppn;    Phytophthora parasitica var. nicotianae;    PR;    pathogenesis-related;    SA;    salicylic acid;    SAR;    systemic acquired resistance;    SDS;    sodium dodecyl sulfate;    SSC;    sodium salt citrate;    tcI;    tobacco cryptogein induced;   

DOI  :  10.1016/S0014-5793(00)01084-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We previously isolated, by differential display and 5′ RACE (rapid amplification of cDNA ends), cDNAs corresponding to genes activated following cryptogein treatment of tobacco cell suspensions, among them tcI 7 (tcI for obacco ryptogein nduced), a gene encoding a β-subunit of proteasome. Here, we report that tcI 7 was up-regulated in tobacco plants treated with elicitins (cryptogein and parasiticein) that have been shown to induce a systemic acquired resistance (SAR). Moreover, subsequent inoculation of tobacco with the pathogen Phytophthora parasitica var. nicotianae (Ppn) was shown to induce an additional activation of tcI 7 in tobacco plants pretreated with cryptogein. We also showed an up-regulation of tcI 7 by salicylic acid (SA). Moreover, accumulation of tcI 7 transcripts after treatment with cryptogein or with SA only occurred in NahG 9⁻ tobacco plants that do not express the salicylate hydroxylase and thus are able to accumulate SA and develop a SAR. Suppressed accumulation of tcI 7 transcripts in NahG 8⁺ tobacco plants after cryptogein or SA treatment correlated with the loss of SAR. H₂O₂ was also shown to up-regulate tcI 7 in tobacco plants. Using gene walking by PCR we cloned and sequenced the 5′ flanking region of tcI 7 containing hypothetical regulatory sequences, especially myb and NF-κB boxes, that could be responsible for the regulation of tcI 7 by salicylic acid and H₂O₂ respectively.

【 授权许可】

Unknown