FEBS Letters | |
Induction of tcI 7, a gene encoding a β‐subunit of proteasome, in tobacco plants treated with elicitins, salicylic acid or hydrogen peroxide 1 | |
Suty, Lydie1  Blein, Jean-Pierre1  Etienne, Philippe1  Petitot, Anne-Sophie1  Houot, Valérie1  | |
[1] Unité Associée INRA-Université de Bourgogne, Phytopharmacie et Biochimie des Interactions Cellulaires, INRA BV 1540, 21034 Dijon Cedex, France | |
关键词: Elicitin; Hydrogen peroxide; Salicylic acid; Proteasome β-subunit; Systemic acquired resistance; Tobacco; GSP; gene-specific primer; GST; glutathione S-transferase; HR; hypersensitive reaction; nPCR; nested polymerase chain reaction; PAL; phenylalanine ammonia-lyase; PCR; polymerase chain reaction; Ppn; Phytophthora parasitica var. nicotianae; PR; pathogenesis-related; SA; salicylic acid; SAR; systemic acquired resistance; SDS; sodium dodecyl sulfate; SSC; sodium salt citrate; tcI; tobacco cryptogein induced; | |
DOI : 10.1016/S0014-5793(00)01084-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We previously isolated, by differential display and 5′ RACE (rapid amplification of cDNA ends), cDNAs corresponding to genes activated following cryptogein treatment of tobacco cell suspensions, among them tcI 7 (tcI for obacco ryptogein nduced), a gene encoding a β-subunit of proteasome. Here, we report that tcI 7 was up-regulated in tobacco plants treated with elicitins (cryptogein and parasiticein) that have been shown to induce a systemic acquired resistance (SAR). Moreover, subsequent inoculation of tobacco with the pathogen Phytophthora parasitica var. nicotianae (Ppn) was shown to induce an additional activation of tcI 7 in tobacco plants pretreated with cryptogein. We also showed an up-regulation of tcI 7 by salicylic acid (SA). Moreover, accumulation of tcI 7 transcripts after treatment with cryptogein or with SA only occurred in NahG 9− tobacco plants that do not express the salicylate hydroxylase and thus are able to accumulate SA and develop a SAR. Suppressed accumulation of tcI 7 transcripts in NahG 8+ tobacco plants after cryptogein or SA treatment correlated with the loss of SAR. H2O2 was also shown to up-regulate tcI 7 in tobacco plants. Using gene walking by PCR we cloned and sequenced the 5′ flanking region of tcI 7 containing hypothetical regulatory sequences, especially myb and NF-κB boxes, that could be responsible for the regulation of tcI 7 by salicylic acid and H2O2 respectively.
【 授权许可】
Unknown
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