The two dimeric forms of RNase A

FEBS Letters

Gotte, Giovanni⁴  Libonati, Massimo⁴  D'Alessio, Giuseppe³  Sorrentino, Salvatore³  Bucci, Enrico²  Barone, Roberto³  Russo, Nello¹ 

[1] Dipartimento di Scienze della Vita, Seconda Università di Napoli, Via Arena 18, 81100 Caserta, Italy;Centro di Studio di Biocristallografia del CNR, Via Mezzocannone 4, 80134 Naples, Italy;Dipartimento di Chimica Organica e Biologica, Università di Napoli Federico II, Via Mezzocannone 16, 80134 Naples, Italy;Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Università di Verona, Strada le Grazie 8, 37134 Verona, Italy

关键词: Ribonuclease;    RNase A;    Protein oligomer;    RNase A;    bovine pancreatic ribonuclease;    D-I;    the prevalent dimer-I fraction of dimeric RNase A;    D-II;    the dimer-II lesser fraction of dimeric RNase A;    BS-RNase;    bovine seminal RNase;    DVS;    divinyl sulfone;    CRI;    cytosolic RNase inhibitor;   

DOI  :  10.1016/S0014-5793(99)01742-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In 1965 Fruchter and Crestfield (J. Biol. Chem. 240, 2868–3874) observed that dimeric RNase A prepared by lyophilization from acetic acid could be separated into two forms. Surprisingly, no other structural or functional differences could be detected between the two forms. In 1998 a structure for dimeric RNase A was determined by X-ray crystallography by Liu et al. (Proc. Natl. Acad. Sci. USA 95, 3437–3442). We found that the two forms of dimeric RNase A have indeed different structural and functional properties, and suggest that the dimer whose structure was investigated by Liu and coworkers may be identified with the lesser form of dimeric RNase A.

【 授权许可】

Unknown