Calumenin interacts with serum amyloid P component

FEBS Letters

Honoré, Bent¹  Jacobsen, Christian¹  Vorum, Henrik¹ 

[1] Department of Medical Biochemistry, Ole Worms Allé, Building 170, University of Aarhus, DK-8000 Aarhus C, Denmark

关键词: Calumenin;    Serum amyloid P component;    Ca2+-dependent interaction;    Low-affinity EF-hand;    CREC protein family;    CRP;    C-reactive protein;    ER;    endoplasmic reticulum;    SAP;    serum amyloid P component;   

DOI  :  10.1016/S0014-5793(99)01734-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We recently reported the identification of human calumenin, a novel Ca²⁺ binding, transformation-sensitive and secreted protein [Vorum et al. (1998) Biochim. Biophys. Acta 1386, 121–131; Vorum et al. (1999) Exp. Cell Res. 248, 473–481] belonging to the family of multiple EF-hand proteins of the secretory pathway that include reticulocalbin, ERC-55, Cab45 and crocalbin. In order to further investigate the extracellular functions of calumenin we immobilized the recombinant protein to a column. After application of a placental tissue extract we were able to elute one protein that interacts with calumenin in the presence of Ca²⁺. Amino acid sequencing identified this protein as serum amyloid P component (SAP). Furthermore, we verified and characterized the calumenin-SAP interaction by the surface plasmon resonance technique. The findings indicate that calumenin may participate in the immunological defense system and could be involved in the pathological process of amyloidosis that leads to formation of amyloid deposits seen in different types of tissues.

【 授权许可】

Unknown