Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP‐dependent protein kinase II

FEBS Letters

Cardone, Luca¹  Garbi, Corrado¹  Ginsberg, Michael D.³  Varrone, Stelio¹  Avvedimento, Enrico V.¹  Feliciello, Antonio¹  Gottesman, Max E.³  Rubin, Charles S.² 

[1] Dipartimento di Biologia e Patologia Molecolare e Cellulare, Centro di Endocrinologia ed Oncologia Sperimentale CNR, Facoltá di Medicina, Universitá Federico II, 80131 Naples, Italy;Department of Molecular Pharmacology, Atran Laboratories, Albert Einstein College of Medicine, Bronx, NY 10461, USA;Institute of Cancer Research, Columbia University, New York, NY 10032, USA

关键词: Yotiao protein;    NMDA receptor;    Protein kinase A;    PKA;    protein kinase A (cAMP-dependent protein kinase);    R;    regulatory subunit of PKA;    C-PKA;    catalytic subunit of PKA;    AKAP;    PKA anchor protein;    NMDA;    N-methyl-D-aspartate;   

DOI  :  10.1016/S0014-5793(99)01585-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A yeast two-hybrid screen revealed that regulatory subunits (RII) of PKAII bind the Yotiao protein. Yotiao interacts with the NR1 subunit of the NMDA receptor. A purified C-terminal fragment of Yotiao binds PKAII, via an RII binding site constituted by amino acid residues 1452–1469, with a dissociation constant (K _d) between 50 and 90 nM in vitro. A stable complex composed of Yotiao, RII and NR1 was immunoprecipitated from whole rat brain extracts. Immunostaining analysis disclosed that Yotiao, RIIβ and NR1 colocalize in striatal and cerebellar neurons. Co-assembly of Yotiao/PKAII complexes with NR1 subunits may promote cAMP-dependent modulation of NMDA receptor activity at synapses, thereby influencing brain development and synaptic plasticity.

【 授权许可】

Unknown