Prediction of human cDNA from its homologous mouse full‐length cDNA and human shotgun database

FEBS Letters

Suzuki, Harukazu¹  Fukunishi, Yoshifumi¹  Yoshino, Masayasu¹  Hayashizaki, Yoshihide¹  Konno, Hideaki¹ 

[1] Genome Science Laboratory, Tsukuba Lifescience Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan

关键词: Human genome;    Human cDNA;    Exon prediction;    Mouse full-length cDNA;    Shotgun sequence;   

DOI  :  10.1016/S0014-5793(99)01696-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We propose a prediction method for human full-length cDNA by comparing sequence data between human genome shotgun sequence and mouse full-length cDNA. The human genome which is homologous to the mouse full-length cDNA is selected by a homology search program, and the predicted exons are connected at the exon-intron junction which gives the best homology score to the mouse full-length cDNA. The accuracy of the predicted human full-length coding region is 83.3%, and the false positive rate is 16.7%. Five human full-length proteins out of 20 proteins are correctly predicted.

【 授权许可】

Unknown