Different induction of gulonolactone oxidase in aromatic hydrocarbon‐responsive or ‐unresponsive mouse strains

FEBS Letters

Braun, László¹  Kardon, Tamás¹  Koulali, Karim El¹  Csala, Miklós¹  Bánhegyi, Gábor¹  Mandl, József¹ 

[1] Department of Medical Chemistry, Semmelweis University of Medicine, P.O. Box 260, H-1444 Budapest, Hungary

关键词: Ascorbate;    Gulonolactone oxidase;    Aromatic hydrocarbon receptor;    Glucuronide;    3-Methylcholanthrene;    Ah;    aromatic hydrocarbon;    AhR;    aromatic hydrocarbon receptor;    Arnt;    AhR nuclear translocator;    B6;    C57BL/6 Ah-responsive mice;    CYP1A1;    cytochrome P-450 1A1;    D2;    DBA/2 Ah-unresponsive mice;    EROD;    7-ethoxyresorufin O-deethylase;    GLO;    L-gulono-γ-lactone oxidase;    3-MC;    3-methylcholanthrene;    MOPS;    4-morpholinepropanesulfonic acid;    UGT1A6;    UDP-glucuronosyltransferase 1A6;   

DOI  :  10.1016/S0014-5793(99)01649-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of aromatic hydrocarbon receptor (AhR)-mediated signal transduction pathways was investigated in the regulation of ascorbate synthesis by using Ah-responsive and Ah-unresponsive mouse strains. In vivo 3-methylcholanthrene treatment increased hepatic and plasma ascorbate concentrations only in the Ah-responsive strain. The mRNA level of gulonolactone oxidase and the microsomal ascorbate production from p-nitrophenyl glucuronide, D-glucuronic acid or gulonolactone in the liver of Ah-responsive and Ah-unresponsive mice were compared. In Ah-responsive mice, these parameters were higher originally, and they further increased upon in vivo addition of 3-methylcholanthrene, while in Ah-unresponsive mice the treatment was not effective. These results suggest that the transcription of gulonolactone oxidase gene is regulated by an Ah receptor-dependent signal transduction pathway.

【 授权许可】

Unknown