N‐terminus of mature heat‐labile enterotoxin chain B is critical for its extracellular secretion in Vibrio cholerae

FEBS Letters

Mukhija, Reema¹  Garg, Lalit C¹ 

[1] Gene Regulation Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India

关键词: Heat-labile enterotoxin chain B;    Extracellular milieu;    Periplasmic expression;    Secretory expression;    N-terminus;   

DOI  :  10.1016/S0014-5793(99)01504-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of addition of a few amino acids to the amino- and carboxy-terminal regions of the mature portion of the heat-labile enterotoxin chain B (LTB) of Escherichia coli on protein export, secretion and assembly were investigated. In E. coli, LTB (secretory protein) with or without the extension at the N- or C-terminus accumulated in the periplasmic fraction. For Vibrio cholerae, LTB with the extension at the C-terminus was exported to the periplasm followed by secretion to the extracellular milieu. However, LTB with the N-terminus extension was exported to the periplasm only. Our findings suggest that in the case of V. cholerae, the N-terminus of the mature LTB plays an important role in its secretion to the extracellular milieu.

【 授权许可】

Unknown