(−)‐Epigallocatechin gallate inhibits Mos activation‐mediated Xenopus oocyte maturation induced by progesterone

FEBS Letters

Ogura, Kumiko¹  Yasokawa, Masayo¹  Iwashita, Jun³  Sugimoto, Isamu¹  Goto, Natsuko¹  Nagata, Katsumi²  Hiji, Yasutake²  Fukuma, Motoyo¹  Kobayashi, Naoki¹  Yasuda, Hiroko¹  Hashimoto, Eikichi¹ 

[1] Department of Pathological Biochemistry, School of Life Sciences, Yonago 683-8503, Japan;First Department of Physiology, Faculty of Medicine, Tottori University, 86 Nishicho, Yonago 683-8503, Japan;Department of Biology, Faculty of Science, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan

关键词: (−)-Epigallocatechin gallate;    Oocyte maturation;    Mos;    Mitogen-activated protein kinase;    Progesterone;    EGCG;    (−)-epigallocatechin gallate;    PKA;    protein kinase A;    PKC;    protein kinase C;    MBP;    myelin basic protein;    rsk;    ribosomal protein S6 kinase;    GVBD;    germinal vesicle break-down;   

DOI  :  10.1016/S0014-5793(99)01646-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

(−)-Epigallocatechin gallate (EGCG), one of the constituents of green tea known to have a tumor preventing effect, inhibited maturation of Xenopus laevis oocytes induced by progesterone when this polyphenol was microinjected into oocytes at a final concentration of about 1 mM. Western blot and activity measurement analyses showed that Mos translation and the subsequent activations of mitogen-activated protein kinase and p90^rsk, probably by protein phosphorylation, seemed to have been inhibited by the microinjection of EGCG. These results suggest that EGCG may have the ability to control Xenopus oocyte maturation at least during the stage of Mos activation.

【 授权许可】

Unknown