Structure of influenza haemagglutinin at neutral and at fusogenic pH by electron cryo‐microscopy

FEBS Letters

Böttcher, Christoph¹  Herrmann, Andreas²  Stark, Holger³  van Heel, Marin⁴  Ludwig, Kai² 

[1] Freie Universität Berlin, Institut für Chemie/Forschungszentrum für Elektronenmikroskopie, Fabeckstr. 36a, D-14195 Berlin, Germany;Humboldt-Universität zu Berlin, Math.-Nat. Fak. I, Institut für Biologie/Biophysik, Invalidenstr. 43, D-10115 Berlin, Germany;Fritz-Haber-Institut, Max-Planck-Gesellschaft, Faradayweg 4-6, D-14195 Berlin, Germany;Imperial College of Science, Medicine and Technology, Wolfson Laboratories, Department of Biochemistry, London SW7 2AY, UK

关键词: Electron microscopy;    Fusion;    Influenza;    Hemagglutinin;    Structure;   

DOI  :  10.1016/S0014-5793(99)01475-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The three-dimensional structures of the complete haemagglutinin (HA) of influenza virus A/Japan/305/57 (H2N2) in its native (neutral pH) and membrane fusion-competent (low pH) form by electron cryo-microscopy at a resolution of 10 Å and 14 Å, respectively, have been determined. In the fusion-competent form the subunits remain closely associated preserving typical overall features of the trimeric ectodomain at neutral pH. Rearrangements of the tertiary structure in the distal and the stem parts are associated with the formation of a central cavity through the entire ectodomain. We suggest that the cavity is essential for relocation of the so-called fusion sequence of HA towards the target membrane.

【 授权许可】

Unknown