Forssman pentasaccharide and polyvalent Galβ1→4GlcNAc as major ligands with affinity for Caragana arborescens agglutinin

FEBS Letters

Wu, June H.²  Wu, Albert M.¹  Herp, Anthony¹  Chen, Yuen-Yuen¹  Tsai, Ming-Sung¹ 

[1] Glyco-Immunochemistry Research Lab., Institute of Molecular and Cellular Biology, College of Medicine, Chang-Gung University, Kwei-San, Tao-Yuan, Taiwan;Department of Microbiology and Immunology, College of Medicine, Chang-Gung University, Kwei-San, Tao-Yuan, Taiwan

关键词: Carbohydrate specificity;    Forssman pentasaccharide;    Caragana arborescens agglutinin;    gp;    glycoprotein;    THGP;    Tamm-Horsfall glycoprotein;    BN;    bird nest glycoprotein;    OSM;    ovine submandibular glycoprotein (major);    RSL;    rat sublingual glycoprotein (major);    ELLSA;    enzyme linked lectinosorbent assay;    Sugars: Gal;    D-galactopyranose;    Glc;    D-glucopyranose;    LFuc;    L-fucopyranose;    GalNAc;    2-acetamido-2-deoxy-D-galactopyranose;    GlcNAc;    2-acetamido-2-deoxy-D-glucopyranose;    Lectin: CAA;    Caragana arborescens agglutinin;    Applied lectin determinants [36–38]: Fp;    GalNAcα1→3GalNAc β1→3Gal α1→4Galβ1→4Glc;    II;    Galβ1→4GlcNAc;    mII;    poly(multi)valent II;    including bi-;    tri- and tetra-antennary and cluster form of II;    Tn;    GalNAcα1→Ser/Thr;    Tri-II;    tri-antennary Galβ1→4GlcNAc:;   

DOI  :  10.1016/S0014-5793(99)01629-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding properties of Caragana arborescens agglutinin (CAA, pea tree agglutinin) were studied by enzyme linked lectinosorbent assay (ELLSA) and by inhibition of CAA-glycan interaction. Among glycoproteins (gps) tested, CAA reacted strongly with asialo bird nest gp, asialo rat sublingual gp, human Tamm-Horsfall Sd(a⁺) urinary gp (THGP) and asialo THGP that are rich in GalNAcα1→, GalNAcβ1→ and/or Galβ1→4GlcNAc residues. CAA also bound tightly with multi-valent Galβ1→4GlcNAc (mII) containing glycoproteins (human blood group precursor gps, asialo fetuin) and asialo ovine salivary glycoprotein (Tn, GalNAcα1→Ser/Thr), but CAA reacted poorly or not at all with sialylated glycoproteins tested. Of the sugars tested for inhibition of binding, Forssman pentasaccharide (F_p, GalNAcα1→3GalNAcβ1→3Galα1→4Galβ1→4Glc) was the best. It was about 2.3, 9.5 and 52.6 times more active than Galβ1→4GlcNAc, GalNAc and Gal, respectively, and about 1.9 times more active than tri-antennary Galβ1→4GlcNAc (Tri-II). These results suggest that this agglutinin is mainly specific for F_p, mII and Tn clusters. This property can be used to detect human abnormal glycotopes related to F_p and unmasked mII/Tn clusters and to study cell growth and differentiation given the lack of toxicity of this lectin toward mouse fibroblast cells.

【 授权许可】

Unknown