Molecular cloning and biological activity of ecdysis‐triggering hormones in Drosophila melanogaster

FEBS Letters

Park, Yoonseong¹  Zitnan, Dusan¹  Gill, Sarjeet S.²  Adams, Michael E.¹ 

[1] Department of Entomology, 5419 Boyce Hall, University of California, Riverside, CA 92521, USA;Department of Neuroscience, 5419 Boyce Hall, University of California, Riverside, CA 92521, USA

关键词: Ecdysis;    Eclosion;    Peptide;    Hormone;    Ecdysis-triggering hormone;    Behavior;    Drosophila;    ETH;    ecdysis-triggering hormone;    CNS;    central nervous system;    EH;    eclosion hormone;    PETH;    pre-ecdysis triggering hormone;    CCAP;    crustacean cardioactive peptide;    PCR;    polymerase chain reaction;    RT-PCR;    reverse transcriptase polymerase chain reaction;    EcRE;    ecdysteroid response element;   

DOI  :  10.1016/S0014-5793(99)01622-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ecdysis-triggering hormones (ETH) initiate a defined behavioral sequence leading to shedding of the insect cuticle. We have identified eth, a gene encoding peptides with ETH-like structure and biological activity in Drosophila melanogaster. The open reading frame contains three putative peptides based on canonical endopeptidase cleavage and amidation sites. Two of the predicted peptides (DrmETH1 and DrmETH2) prepared by chemical synthesis induce premature eclosion upon injection into pharate adults. The promoter region of the gene contains a direct repeat ecdysteroid response element. Identification of eth in Drosophila provides opportunities for genetic manipulation of endocrine and behavioral events underlying a stereotypic behavior.

【 授权许可】

Unknown