Simultaneous core 2 β1→6N‐acetylglucosaminyltransferase up‐regulation and sialyl‐LeX expression during activation of human tonsillar B lymphocytes

FEBS Letters

Nakamura, Mitsuru²  Furukawa, Yusuke²  Matsuda, Michio²  Ishida, Takashi¹  Kikuchi, Jiro² 

[1] Department of Oto-naso-laryngology, Jichi Medical School, Minamikawachi, Tochigi 329-0498, Japan;Division of Molecular Hemopoiesis, Center for Molecular Medicine, Jichi Medical School, Minamikawachi, Tochigi 329-0498, Japan

关键词: Sialyl-LeX;    B cell activation;    Core 2 GlcNAc transferase;    Tonsillar B cell;    Fucosyltransferase;    α2→3-sialyltransferase and β1→4-galactosyltransferases are designated according to the recommendations [1–3];    sLeX;    sialylated Lewis antigen with Galβ1→4GlcNAc backbone;    C2GnT;    core 2 β1→6GlcNAc transferase or UDP-GlcNAc:Galβ1→3GalNAc (GlcNAc to GalNAc) β1→6-N-acetylglucosaminyltransferase;    PWM;    pokeweed mitogen;    Bz-α-GalNAc;    benzyl-α-GalNAc;    PDMP;    d-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol;    dThd;    thymidine;   

DOI  :  10.1016/S0014-5793(99)01619-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have investigated the regulation mechanism of the surface sialyl-Le^X (sLe^X) expression level in tonsillar B cells during activation. sLe^X antigen became strongly positive after activation, while resting B cells were weakly positive. sLe^X structures were mainly located on O-linked oligosaccharide chains of glycoprotein. Transcripts of FucT-VII and core 2 GlcNAc transferase (C2GnT) were up-regulated after activation, while those of ST3GalIV and β1→4GalT-I were expressed constitutively. However, the up-regulation of C2GnT was more dramatic than that of FucT-VII. These results suggest that sLe^X expression level is regulated by C2GnT during tonsillar B cell activation.

【 授权许可】

Unknown