Hypertrophic stimuli augment expression of cMG1/ERF‐1, a putative zinc‐finger motif transcription factor, in rat cardiomyocytes

FEBS Letters

Ogawa, Satoshi¹  Pan, Jing¹  Yamaguchi, Ken²  Fukuda, Keiichi¹  Manabe, Tomohiro¹  Nagasaki, Koichi² 

[1] Cardiopulmonary Division, Department of Internal Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan;Growth Factor Division, National Cancer Center Research Institute, 5-1-1, Tsukiji, Chuo-ku, Tokyo 104-0045, Japan

关键词: Leukemia inhibitory factor;    cMG1/ERF-1;    Cardiac hypertrophy;    Rat cardiomyocyte;    LIF;    leukemia inhibitory factor;    JAK;    Janus kinase;    ET-1;    endothelin-1;    AngII;    angiotensin II;    DD;    differential display;    ERF-1;    EGF-response factor 1;    STAT;    signal transducers and activators of transcription;   

DOI  :  10.1016/S0014-5793(99)01601-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We isolated the gene for cMG1/ERF-1, a known putative zinc-finger transcription factor, by differential display of mRNA extracted from cardiomyocytes with and without leukemia inhibitory factor (LIF) stimulation. LIF induced cMG1/ERF-1 mRNA at 15 min, and levels peaked at 10-fold initial levels at 30 min. cMG1/ERF-1 expression was inhibited by AG490 (JAK2 inhibitor) and genistein, but was unaffected by PD98059 or wortmannin. Phenylephrine, angiotensin II and endothelin-1 also induced cMG1/ERF-1 expression. Mechanical stretch in vitro and acute pressure overload in vivo increased cMG1/ERF-1 expression. To our knowledge, this is the first report showing that the cMG1/ERF-1 gene can be induced by various hypertrophic stimuli, and that Janus kinase 2 is involved in this process.

【 授权许可】

Unknown