Somatostatin receptor subtype 1 modulates basal inhibition of growth hormone release in somatotrophs

FEBS Letters

Kreienkamp, Hans-Jürgen¹  Akgün, Ercan¹  Meyerhof, Wolfgang²  Richter, Dietmar¹  Baumeister, Hans² 

[1] Institut für Zellbiochemie und klinische Neurobiologie, Universität Hamburg, Martinistrasse 52, D-20246 Hamburg, Germany;Deutsches Institut für Ernährungsforschung Potsdam-Rehbrücke, Arthur-Scheunert-Allee 114-116, D-14558 Bergholz-Rehbrücke, Germany

关键词: SSTR1 null mutation;    Growth hormone release;    SSTR;    somatostatin receptor;    SST;    somatostatin;    ES cells;    embryonic stem cells;    CH-275;    [des-Ala1;    des-Gly2;    des-Asn5;    D-Trp8;    IAmp9] somatostatin-14;    GH;    growth hormone;    BSA;    bovine serum albumin;    PBS;    phosphate buffered saline;    DMEM;    Dulbecco's modified Eagle medium;    SST-14;    somatostatin-14;    GAPDH;    glycerine aldehyde phospho dehydrogenase;    ko;    knock out;    wt;    wild-type;   

DOI  :  10.1016/S0014-5793(99)01582-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Somatostatin (SST) inhibits the secretion of many peptide hormones including growth hormone (GH). The various functions of SST are mediated through at least five different receptor subtypes (SSTR1–5), their precise physiological roles have not been solved yet. Here we report on studies concerning the functional role of SSTR1 in the modulation of GH release from somatotrophs. Primary cell cultures from pituitaries of wild-type SSTR1 mice exposed to the SSTR1 selective somatostatin analog CH-275 show reduction of basal levels of GH secretion whereas somatotrophs isolated from SSTR1 null mutant mice did not respond to the agonist-mediated effect. This suggests that SSTR1 is involved in modulating basal GH levels in primary pituitary cell cultures and, together with SSTR2, may control the secretion of GH in the body.

【 授权许可】

Unknown