FEBS Letters | |
Functional properties of complement factor H‐related proteins FHR‐3 and FHR‐4: binding to the C3d region of C3b and differential regulation by heparin | |
Hellwage, Jens2  Jokiranta, T.Sakari2  Zipfel, Peter F.2  Meri, Seppo3  Vaarala, Outi1  Koistinen, Vesa3  | |
[1] Department of Biochemistry, National Public Health Institute, Mannerheimintie 166, FIN-00300 Helsinki, Finland;Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, D-20359 Hamburg, Germany;Haartman Institute and HD-Diagnostics, Department of Bacteriology and Immunology, Haartmaninkatu 3, FIN-00290 Helsinki, Finland | |
关键词: Complement regulation; Heparin binding; Surface plasmon resonance; Alternative pathway; Streptococcus pneumoniae; FH; complement factor H; FHDS; factor H-deficient serum; FHR-3; factor H-related protein 3; FHR-4; factor H-related protein 4; SCR; short consensus repeat; | |
DOI : 10.1016/S0014-5793(99)01554-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The human factor H-related proteins FHR-3 and FHR-4 are members of a family of proteins related to the complement factor H. Here, we report that the two proteins bind to the C3d region of complement C3b. The apparent K A values for the interactions of FHR-3 and FHR-4 with C3b are 7.5×106 M−1 and 2.9×106 M−1, respectively. Binding studies performed with C3b-coated pneumococci confirmed the results obtained with the biosensor system. A C-terminal construct of factor H showed similar binding characteristics. The interaction of FHR-3, but not of FHR-4, with opsonised pneumococci was inhibited by heparin.
【 授权许可】
Unknown
【 预 览 】
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