A new matrix protein family related to the nacreous layer formation of Pinctada fucata

FEBS Letters

Hayashi, Nakanobu¹  Horita, Chie²  Hasegawa, Kyoko⁴  Kono, Makiko³  Akera, Syusaku²  Samata, Tetsuro³ 

[1]Omgen Inc., Itabashi, Tokyo 173-0031, Japan

[2]Tasaki Institute for Marine Biological Research, Hiwasa, Tokushima 779-23, Japan

[3]Laboratory of Cell Biology, Faculty of Environmental Health, Azabu University, 1-17-71 Fuchinobe, Sagamihara 229-8501, Japan

[4]New Japan Alfa Inc., Atsugi, Kanagawa 243-0811, Japan

关键词: Biomineralization;    Nacreous layer;    Organic matrix;    cDNA cloning;    Crystallization experiment;   

DOI  :  10.1016/S0014-5793(99)01387-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated a new matrix protein family (N16) which is specific to the nacreous layer of the Japanese pearl oyster, Pinctada fucata, and have cloned and characterized the cDNAs coding for the components. Analysis of the deduced amino acid sequence revealed that N16 showed no definitive homology with other proteins. The in vitro studies of the crystallization clarified that N16 induced aragonite crystals when fixed on the substrate but inhibited crystal formation without it. The aragonite crystals showed platy morphology different from those formed inorganically, and long intervals of incubation resulted in crystalline layers highly similar to the nacreous layer.

【 授权许可】

Unknown