Mannan‐binding lectin (MBL)‐associated plasma protein present in human urine inhibits calcium oxalate crystal growth

FEBS Letters

Ha, Joohun¹  Lee, Sun-Ju²  Choi, Sang-Lim¹  Kang, Insug¹  Kim, Sung Soo¹  Kim, Jin-Il²  Chang, Sung-Goo² 

[1] Department of Molecular Biology, School of Medicine, Kyung Hee University, # 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, South Korea;Department of Urology, School of Medicine, Kyung Hee University, Seoul 130-701, South Korea

关键词: Mannan-binding lectin-associated plasma protein;    Human urine;    Renal cell carcinoma;    Calcium oxalate crystal;    Renal stone;    MBL;    mannan-binding lectin;    MAp19;    MBL-associated plasma protein;    MASP;    MBL-associated serine protease;    DEAE;    diethylaminoethyl;    IPTG;    isopropyl-β-thiogalactoside;    HPLC;    high performance liquid chromatography;    EGF;    epidermal growth factor;   

DOI  :  10.1016/S0014-5793(99)01509-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mannan-binding lectin (MBL)-associated plasma protein (MAp19) is an alternatively spliced form of MBL-associated serine protease-2, a component of a complement activation cascade. We observed that MAp19 is excreted in human urine. Interestingly, the amount of MAp19 was higher in urine of renal cell carcinoma patients than healthy people. Pretreatment of urine dialysate with 50 mM EDTA increased the recovery of MAp19, suggesting that MAp19 is a calcium-binding protein. The recombinant MAp19 showed a strong inhibition of calcium oxalate crystal growth in vitro in a concentration-dependent manner. Thus, we conclude that MAp19 plays a role in the inhibition of calcium oxalate renal stone formation.

【 授权许可】

Unknown