Static and magic angle spinning 31P NMR spectroscopy of two natural plasma membranes

FEBS Letters

Le Floch, M.²  Moreau, C.³  Segalen, J.³  Le Rumeur, E.³  de Certaines, J.D.³  Leray, G.³  Metzinger, L.¹ 

[1] Biochimie Pharmaceutique, Faculté de Pharmacie, 35043 Rennes, France;UMR CNRS 6512, Faculté des Sciences, 263 avenue du Général Leclerc, 35042 Rennes, France;Laboratoire de RMN en Biologie et Médecine (LRMBM), Faculté de Médecine, CS 34317, 35043 Rennes, France

关键词: 31P NMR spectroscopy;    Magic angle spinning;    Plasma membrane;    Phospholipid composition;    Bilayer arrangement;   

DOI  :  10.1016/S0014-5793(99)01461-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Static and magic angle spinning ³¹P NMR spectroscopy was used for the first time in natural plasma membranes from erythrocytes and skeletal muscle to study phospholipid arrangement and composition. Typical static powder-like spectra were obtained showing that phospholipids were in a bilayer arrangement. Magic angle spinning narrowed spectra into two components. The first one corresponded to phosphatidylcholine and the second one to the other phospholipids with intensities in agreement with the known phospholipid composition. These findings show that NMR data previously acquired using model membranes can be transposed to studies on phospholipids in their natural environment.

【 授权许可】

Unknown