Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants

FEBS Letters

Shimada, Yukihisa³  Ohbayashi, Masaya⁴  Kikuchi, Yasuhiro⁴  Okinaka, Yasushi¹  Kiyokawa, Shigeto²  Nakano-Shimada, Ritsuko³ 

[1] Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto 606-01, Japan;Department of Bioscience and Biotechnology, Aomori University, Kobata 2-3-1, Aomori 030-0943, Japan;Plant Functions Laboratory, The Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan;Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-city, Tokyo 194-8533, Japan

关键词: Anthocyanin biosynthesis;    Flower color;    Flavonoid-3′;    5′-hydroxylase;    P450;    Transgenic plant;    Electron transport system for P450;    F3′5′H;    flavonoid-3′;    5′-hydroxylase;    F3′H;    flavonoid-3′-hydroxylase;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(99)01425-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Flavonoid-3′,5′-hydroxylase (F3′5′H), a member of the cytochrome P450 family, is the key enzyme in the synthesis of 3′,5′-hydroxylated anthocyanins, which are generally required for blue or purple flowers. A full-length cDNA, TG1, was isolated from prairie gentian by heterologous hybridization with a petunia cDNA, AK14, which encodes F3′5′H. To investigate the in vivo function of TG1 and AK14, they were subcloned into a plant expression vector and expressed under the control of the CaMV35S promoter in transgenic tobacco or petunia, both of which originally lack the enzyme. Transgenic petunia plants had a dramatic change in flower color from pink to magenta with a high content of 3′,5′-hydroxylated anthocyanins. In contrast, transgenic tobacco plants had minimal color change with at most 35% 3′,5′-hydroxylated anthocyanin content. These results indicate that the products of TG1 and AK14 have F3′5′H activity in planta and that interspecific gene transfer alters anthocyanin pigment synthesis. The difference in apparent F3′5′H activity between tobacco and petunia is discussed.

【 授权许可】

Unknown