Antisense suppression of proline degradation improves tolerance to freezing and salinity in Arabidopsis thaliana

FEBS Letters

Kakubari, Yoshitaka¹  Kobayashi, Masatomo³  Yoshiba, Yoshu²  Nanjo, Tokihiko³  Yamaguchi-Shinozaki, Kazuko⁴  Shinozaki, Kazuo³ 

[1] Department of Forestry and Forest Resources, Faculty of Agriculture, University of Shizuoka, 836 Oya, Shizuoka 422-8529, Japan;Life Science Research Center, Central Research Laboratory, Hitachi Ltd., Hatoyama, Saitama 350-0395, Japan;Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan;Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Ministry of Agriculture, Forestry and Fisheries, 2-1 Ohwashi, Tsukuba, Ibaraki 305-0851, Japan

关键词: Antisense;    Proline dehydrogenase;    Freezing tolerance;    Salinity tolerance;    Hydroponic culture;    Pro;    proline;    P5C;    Δ1-pyrroline-5-carboxylate;    GSA;    glutamic-γ-semialdehyde;    P5CS;    P5C synthase;    P5CR;    P5C reductase;    ProDH;    proline dehydrogenase;    P5CDH;    P5C dehydrogenase;   

DOI  :  10.1016/S0014-5793(99)01451-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synthesis, degradation, and transport of proline (Pro) are thought to cooperatively control its endogenous levels in higher plants in response to environmental conditions. To evaluate the function of Pro degradation in the regulation of the levels of Pro and to elucidate roles of Pro in stress tolerance, we generated antisense transgenic Arabidopsis plants with an AtProDH cDNA encoding proline dehydrogenase (ProDH), which catalyzes Pro degradation. Several transgenic lines accumulated Pro at higher levels than wild-type plants, providing evidence for a key role of ProDH in Pro degradation in Arabidopsis. These antisense transgenics were more tolerant to freezing and high salinity than wild-type plants, showing a positive correlation between Pro accumulation and stress tolerance in plants.

【 授权许可】

Unknown