The importance of C‐terminal residues of vertebrate and invertebrate tachykinins for their contractile activities in gut tissues

FEBS Letters

Ikeda, Tetsuya¹  Minakata, Hiroyuki¹  Nomoto, Kyosuke¹ 

[1] Suntory Institute for Bioorganic Research, Shimamotocho, Osaka 618-8503, Japan

关键词: Invertebrate tachykinin;    Urechistachykinin;    C-terminal residue;    Cockroach hindgut;    Guinea pig ileum;   

DOI  :  10.1016/S0014-5793(99)01457-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The C-terminal residues of mammalian tachykinins and urechistachykinins (Uru-TKs), tachykinin-related peptides of echiuroid worm origin, were substituted for each other. Their contractile effects were assayed on the cockroach hindgut and the guinea pig ileum. [Met¹⁰] substitution of Uru-TKs caused a 1000 times lower activity on the hindgut, but a 1000 times higher activity on the ileum. In contrast, [Arg¹¹]substance P (SP) was 100 times more and 400 times less potent than SP on the hindgut and ileum, respectively. A SP antagonist blocked these Uru-TK activities on the hindgut. These results demonstrated that the C-terminal Met-NH₂ is necessary for ileum contraction and the Arg-NH₂ is required for hindgut contraction, which was caused by binding to the cockroach's neurokinin-like receptor.

【 授权许可】

Unknown