Characterisation of the binding interaction between poly(L‐lysine) and DNA using the fluorescamine assay in the preparation of non‐viral gene delivery vectors

FEBS Letters

Read, Martin L.¹  Seymour, Leonard W.¹  Ulbrich, Karel²  Etrych, Thomas² 

[1] CRC Institute for Cancer Studies, University of Birmingham, Birmingham B15 2TT, UK;Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague 6, Czech Republic

关键词: DNA;    Gene delivery;    Gene therapy;    Fluorescamine;    Polyelectrolyte complex;    IPEC;    inter-polyelectrolyte complex;    pLL;    poly(L-lysine);    pEG;    poly(ethylene glycol);    pHPMA-ONp;    poly(N-(2-hydroxypropyl)methacrylamide);    CT DNA;    calf thymus DNA;   

DOI  :  10.1016/S0014-5793(99)01435-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A major factor limiting the development of non-viral gene delivery systems is the poor characterisation of polyelectrolyte complexes formed between cationic polymers and DNA. The present study uses the fluorescamine reagent to improve characterisation of poly(L-lysine) (pLL)/DNA complexes post-modified with a multivalent hydrophilic polymer by determining the availability of free amino groups. The results show that the fluorescamine reagent can be used to monitor the self-assembly reaction between pLL and DNA and the degree of surface modification of the resultant complexes with a hydrophilic polymer. This experimental approach should enable the preparation of fully defined complexes whose properties can be better related to their biological activity.

【 授权许可】

Unknown