FEBS Letters | |
The plasmid F OmpP protease, a homologue of OmpT, as a potential obstacle to E. coli‐based protein production | |
Matsuo, Ei-ichi1  Mizobuchi, Kiyoshi2  Sampei, Gen-ichi1  Ito, Koreaki1  | |
[1] Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan;Department of Applied Physics and Chemistry, University of Electro-Communications, Chofu-shi, Tokyo 182-8585, Japan | |
关键词: OmpT; SecY; OmpP; F plasmid; Recombinant protein; Escherichia coli; | |
DOI : 10.1016/S0014-5793(99)01418-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
OmpT, an outer membrane-localized protease of Escherichia coli, cleaves a number of exogenous and endogenous proteins during their purification. SecY, an endogenous membrane protein, is a target of this artificial proteolysis in vitro. Here we report that SecY cleavage occurs even in cell extracts from ompT-disrupted cells, if they carry an F plasmid derivative. A gene, ompP, on the F plasmid was shown to be responsible for this proteolysis. These results indicate that the absence of an F-like plasmid should be checked when choosing a host strain for E. coli-based protein production.
【 授权许可】
Unknown
【 预 览 】
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RO201912020308545ZK.pdf | 109KB | download |