The plasmid F OmpP protease, a homologue of OmpT, as a potential obstacle to E. coli‐based protein production

FEBS Letters

Matsuo, Ei-ichi¹  Mizobuchi, Kiyoshi²  Sampei, Gen-ichi¹  Ito, Koreaki¹ 

[1] Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan;Department of Applied Physics and Chemistry, University of Electro-Communications, Chofu-shi, Tokyo 182-8585, Japan

关键词: OmpT;    SecY;    OmpP;    F plasmid;    Recombinant protein;    Escherichia coli;   

DOI  :  10.1016/S0014-5793(99)01418-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

OmpT, an outer membrane-localized protease of Escherichia coli, cleaves a number of exogenous and endogenous proteins during their purification. SecY, an endogenous membrane protein, is a target of this artificial proteolysis in vitro. Here we report that SecY cleavage occurs even in cell extracts from ompT-disrupted cells, if they carry an F plasmid derivative. A gene, ompP, on the F plasmid was shown to be responsible for this proteolysis. These results indicate that the absence of an F-like plasmid should be checked when choosing a host strain for E. coli-based protein production.

【 授权许可】

Unknown