Effect of sphingosylphosphorylcholine on the single channel gating properties of the cardiac ryanodine receptor

FEBS Letters

Uehara, Akira¹  Yasukochi, Midori²  Berlin, Joshua R.³  Imanaga, Issei¹ 

[1] Department of Physiology, School of Medicine, Fukuoka University, 45-1, 7-chome Nanakuma, Jonan-ku, Fukuoka 814-80, Japan;Laboratory of Human Biology, School of Medicine, Fukuoka University, 45-1, 7-chome Nanakuma, Jonan-ku, Fukuoka 814-80, Japan;Department of Pharmacology/Physiology, UMDNJ-New Jersey Medical School, 185 S. Orange Ave., Newark, NJ 07103, USA

关键词: Heart;    Ca2+ release;    Ryanodine receptor;    Sphingolipid;    Single channel;    Lipid bilayer;   

DOI  :  10.1016/S0014-5793(99)01385-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of the lysosphingolipid, sphingosylphosphorylcholine (SPC), on the cardiac ryanodine receptor (RyR) were examined. The open probability of cardiac RyR incorporated in lipid bilayers was decreased by cytoplasmic, but not lumenal side application of micromolar concentrations of SPC. Modification of channel function was characterized by the appearance of a long-lived closed state in addition to the brief channel closings observed in the presence and absence of SPC. Open channel kinetics and ion conduction properties, however, were not altered by this compound. These results suggest that SPC, a putative second messenger derived from sphingomyelin, may regulate Ca²⁺ release from the sarcoplasmic reticulum by modifying the gating kinetics of the RyR.

【 授权许可】

Unknown