The cadherin‐like protein is essential to specificity determination and cytotoxic action of the Bacillus thuringiensis insecticidal CryIAa toxin

FEBS Letters

Nagamatsu, Yasunori²  Sasaki, Kazuhiro²  Yoshimoto, Akihiro²  Furukawa, Yasuo¹  Koike, Takashi² 

[1] Department of Biological Science, Faculty of Science, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8526, Japan;Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739-8528, Japan

关键词: Insecticidal protein;    Receptor;    Cell swelling;    Cadherin-like protein;    Pore formation;    Bacillus thuringiensis;    FITC;    fluorescein isothiocyanate;    HEPES;    2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic acid;   

DOI  :  10.1016/S0014-5793(99)01327-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Bacillus thuringiensis CryIAa toxin binds a cadherin-like protein (BtR175) on the brush-border membranes of the Bombyx mori midgut columnar cells, which are the targets. By introducing the BtR175 gene with a baculovirus, Spodoptera frugiperda Sf9 cells expressed BtR175 protein on the cell membrane and became susceptible to the CryIAa toxin. The toxin bound the cadherin repeat adjacent to the membrane and made a pore that passed inorganic ions, causing the cell to swell and burst. This was not observed with a BtR175 variant lacking the toxin-binding site. This in vitro experiment mimicked the specific insecticidal action of the toxin in vivo well.

【 授权许可】

Unknown