B‐myb proto‐oncogene products interact in vivo with each other via the carboxy‐terminal conserved region

FEBS Letters

Min, Sanghyun²  Ha, Hyunjung²  Kim, Kyong-Tai¹  Kim, Taenam²  Jung, Haiyoung² 

[1] Department of Life Science, Pohang University of Science and Technology, Pohang 790-784, South Korea;Department of Biochemistry and Division of Life Sciences, Chungbuk National University, Cheongju 361-763, South Korea

关键词: B-myb oncogene;    Self-association;    Conserved region;   

DOI  :  10.1016/S0014-5793(99)01375-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using the yeast two-hybrid assay and in vivo binding assay, we investigated whether B-myb oncogene products (B-myb) can associate with each other. Specificity tests of the yeast two-hybrid system showed a self-association of B-myb proteins in yeast. Cotransfection experiments demonstrated that B-myb proteins form a complex in vivo. Deletion analysis revealed that this binding was sufficiently mediated by the carboxy-terminal conserved region of B-myb. In addition, the B-myb self-association is directly dependent on the amount of expressed B-myb in cells and slightly increased by the dephosphorylation state. These results suggested that B-myb could form a complex and influence its transcriptional activity.

【 授权许可】

Unknown