TRAF1 is a TNF inducible regulator of NF‐κB activation

FEBS Letters

Beyaert, Rudi¹  Carpentier, Isabelle¹ 

[1] Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology, University of Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium

关键词: TNF receptor factor;    Nuclear factor κB inhibition;    Tumor necrosis factor;    FADD;    Fas-associated protein with death domain;    IL-1;    interleukin-1;    MAPK;    mitogen activated protein kinase;    NF-κB;    nuclear factor κB;    PKC;    protein kinase C;    PMA;    phorbol myristate acetate;    RIP;    receptor interacting protein;    TNF;    tumor necrosis factor;    TNFR;    TNF receptor;    TRADD;    TNFR1-associated death domain protein;    TRAF;    TNFR associated factor;   

DOI  :  10.1016/S0014-5793(99)01356-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tumor necrosis factor receptor (TNFR)-associated factor TRAF1 was first identified as a component of the TNFR2 signalling complex. Unlike the other members of the TRAF family, TRAF1 lacks the N-terminal ring finger motif and has a tissue specific expression. Here we demonstrate that expression of TRAF1 is induced by TNF and the protein kinase C (PKC) activator PMA, but not by interleukin-1 (IL-1). TNF-induced upregulation of TRAF1 could be prevented by pretreatment of the cells with the proteasome inhibitor MG-132, whereas the PKC inhibitor Ro31-8220 was without effect. Interestingly, overexpression of TRAF1 in HEK293T completely prevented NF-κB activation induced by TNF, IL-1, or overexpression of TRAF2 or TRAF6. These data suggest that inducible expression of TRAF1 may serve a negative regulatory function in NF-κB signalling pathways.

【 授权许可】

Unknown