DNA methylation and chromosome instability in breast cancer cell lines

FEBS Letters

Vogt, Nicolas¹  Malfoy, Bernard¹  Dutrillaux, Bernard¹  Vilain, Armelle¹ 

[1] Institut Curie, UMR 147-CNRS, 26 rue d'Ulm, 75248 Paris, Cedex 05, France

关键词: DNA methylation;    Genome instability;    DNA methyltransferase 1;    DNA demethylase;    Breast cancer;   

DOI  :  10.1016/S0014-5793(99)01358-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We show that in a series of eight breast cancer cell lines, a direct relationship exists between the overall DNA demethylation and the percentage of rearranged chromosomes, except for cell lines with a highly rearranged genome which can be weakly demethylated. A real time fluorescent detection method was used to quantify by reverse transcription-PCR the expression of the DNA methyltransferase 1 and of the newly discovered DNA demethylase. The overall DNA methylation status seems to result from a complex interplay between the expression of these two genes. Our results suggest that in these tumor cells, the overall DNA demethylation is implicated in one of the mechanisms at the origin of the genome instability and that besides the role of the DNA methyltransferase 1, that of the DNA demethylase may be essential in the control of DNA methylation.

【 授权许可】

Unknown