Isolation and characterization of the E2F‐like gene in plants

FEBS Letters

Shinmyo, Atsuhiko¹  Sekine, Masami¹  Uemukai, Kenji¹  Maeda, Yoshio¹  Nakagami, Hirofumi¹  Ito, Masaki² 

[1] Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST), Takayama 8916-5, Ikoma, Nara 630-0101, Japan;Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: E2F;    Cyclin;    Cell cycle;    Transcription factor;    Nicotiana tabacum;    CDK;    cyclin-dependent serine/threonine protein kinase;    Rb;    retinoblastoma;    PCNA;    proliferating cell nuclear antigen;    RT-PCR;    reverse transcription-polymerase chain reaction;    CaMV;    cauliflower mosaic virus;   

DOI  :  10.1016/S0014-5793(99)01296-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The transcription factor E2F regulates the expression of genes involved in the progression of G1/S transition and DNA replication in mammalian cells. We cloned and characterized a cDNA (NtE2F) corresponding to a E2F homolog of tobacco (Nicotiana tabacum). The transcription of NtE2F was induced as cells progressed from G1 to the S phase and expressed much earlier than that of the proliferating cell nuclear antigen (PCNA) gene. We demonstrated that NtE2F can interact with the tobacco retinoblastoma (Rb)-related protein in a yeast two-hybrid assay. To further characterize NtE2F, the trans-activation activity of NtE2F was examined by using a transient assay in the tobacco Bright Yellow-2 (BY-2) cells with NtE2F fused to the DNA-binding domain of the yeast transcriptional activator GAL4. NtE2F activated the transcription of the β-glucuronidase (GUS) reporter gene driven by a cauliflower mosaic virus (CaMV) 35S core promoter containing the GAL4-binding sequence. This is the first report of the identification of a functionally equivalent E2F-like gene in plants.

【 授权许可】

Unknown