Regions in vertebrate photoreceptor guanylyl cyclase ROS‐GC1 involved in Ca2+‐dependent regulation by guanylyl cyclase‐activating protein GCAP‐1

FEBS Letters

Koch, Karl-Wilhelm³  Beyermann, Michael¹  Lange, Christian³  Sharma, Rameshwar K.²  Duda, Teresa² 

[1] Forschungsinstitut für Molekulare Pharmakologie, Alfred-Kowalke-Strasse 4, D-10315 Berlin, Germany;The Unit of Regulatory and Molecular Biology, Departments of Cell Biology and Ophthalmology, NJMS, University of Medicine and Dentistry of New Jersey, Stratford, NJ 08084, USA;Institut für Biologische Informationsverarbeitung-1, Forschungszentrum Jülich, D-52425 Jülich, Germany

关键词: Phototransduction;    Guanylyl cyclase;    Calcium-binding protein;    Guanylyl cyclase-activating protein 1;    GC;    guanylyl cyclase;    ROS;    rod outer segments;    ROS-GC1;    photoreceptor membrane guanylyl cyclase 1;    GCAP;    guanylyl cyclase-activating protein;    KHD;    kinase homology domain;    JMD;    juxtamembrane domain;    GC-A;    atrial natriuretic factor receptor guanylyl cyclase;    HPLC;    high performance liquid chromatography;   

DOI  :  10.1016/S0014-5793(99)01312-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The membrane bound guanylyl cyclase (GC) photoreceptor membrane GC1 (ROS-GC1) of photoreceptor cells synthesizes cGMP, the intracellular transmitter of vertebrate phototransduction. The activity of ROS-GC1 is controlled by small Ca²⁺-binding proteins, named GC-activating proteins (GCAPs). We identified and characterized two short regulatory regions (M445–L456 and L503–I522) in the juxtamembrane domain (JMD) of ROS-GC1 by peptide competition and mutagenesis studies. Both regions are critical for the activation of ROS-GC1 by GCAP-1.

【 授权许可】

Unknown