The yeast Rgd1p is a GTPase activating protein of the Rho3 and Rho4 proteins

FEBS Letters

Doignon, François¹  Weinachter, Caroline¹  Roumanie, Olivier¹  Crouzet, Marc¹ 

[1] Laboratoire de Biologie Moléculaire et de Séquençage, UPR CNRS 9026, BP Box 64, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France

关键词: RHO3;    RHO4;    RGD1;    GTPase activating protein;    Saccharomyces cerevisiae;    GAP;    GTPase activating protein;    GTP;    guanosine 5′-triphosphate;    GST;    glutathione S-transferase;    MESG;    7-methyl 6-thioguanosine;    PCR;    polymerase chain reaction;    Pi;    inorganic phosphate;   

DOI  :  10.1016/S0014-5793(99)01293-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The RGD1 gene, identified during sequencing of the Saccharomyces cerevisiae genome, encodes a protein with a Rho-GTPase activating protein (GAP) domain at the carboxy-terminal end. The Rgd1 protein showed two-hybrid interactions with the activated forms of Rho2p, Rho3p and Rho4p. Using in vitro assays, we demonstrated that Rgd1p stimulated the GTPase activity of both Rho3p and Rho4p; no stimulation was observed on Rho2p. In addition, the rho3Δrgd1Δ double mutant exhibited a dramatic growth defect compared to the single mutants, suggesting that Rgd1p has a GAP activity in vivo. The present study allowed the identification of the first GAP of Rho3p and Rho4p.

【 授权许可】

Unknown