Analysis by atomic force microscopy of Med8 binding to cis‐acting regulatory elements of the SUC2 and HXK2 genes of Saccharomyces cerevisiae

FEBS Letters

Baró, A.M.¹  Moreno-Herrero, F.²  Colchero, J.¹  Moreno, F.²  Herrero, P.² 

[1] Laboratorio de Nuevas Microscopı́as, Departamento de Fı́sica de la Materia Condensada, Universidad Autónoma de Madrid, 28049 Madrid, Spain;Departamento de Bioquı́mica y Biologı́a Molecular, Instituto Universitario de Biotecnologı́a de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain

关键词: Atomic force microscopy;    SUC2;    HXK2;    Med8;    Transcriptional regulation;    Saccharomyces cerevisiae;   

DOI  :  10.1016/S0014-5793(99)01289-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Med8 protein is a regulator that specifically binds to upstream activating sequences (UASs) of SUC2 promoter, to downstream repressing sequences (DRSs) of the HXK2 gene and to the carboxy-terminal domain of the RNA polymerase II. Atomic force microscopy has allowed for direct visualization of Med8 interactions with a 305 bp fragment of SUC2 promoter and with a 676 bp fragment of HXK2 gene, containing respectively the UASs and DRSs regulatory regions. This approach has provided complementary information about the position and the structure of the DNA-protein complexes. Med8 binding to DNA results in total covering of one of the two existing 7 bp motives (consensus, (A/C)(A/G)GAAAT) in the studied DNA fragments. No preference for binding either of the two UASs of SUC2 promoter as well as for the two DRSs of HXK2 gene has been found. We also discuss whether this protein works as dimer or as a monomer.

【 授权许可】

Unknown