Demonstration of glycosaminoglycans in Caenorhabditis elegans

FEBS Letters

Yokota, Akiko¹  Van Die, Irma²  Van den Eijnden, Dirk H.²  Kitagawa, Hiroshi¹  Sugahara, Kazuyuki¹  Yamada, Shuhei¹ 

[1] Department of Biochemistry, Kobe Pharmaceutical University, 4-19-1 Motoyamakita-machi, Higashinada-ku, Kobe 658-8558, Japan;Department of Medical Chemistry, Vrije Universiteit, 1081 BT Amsterdam, The Netherlands

关键词: Chondroitin;    Heparan sulfate;    Glycosaminoglycan;    1H NMR;    Caenorhabditis elegans;    2-AB;    2-aminobenzamide;    ΔHexA;    4-deoxy-α-L-threo-hex-4-enepyranosyluronic acid;    GlcA;    D-glucuronic acid;    IdceA;    L-iduronic acid;    CS;    chondroitin sulfate;    HS;    heparan sulfate;    GAG;    glycosaminoglycan;    HPLC;    high-performance liquid chromatography;   

DOI  :  10.1016/S0014-5793(99)01286-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A considerable amount (approximately 1.6 μg from 1 mg of dried nematode) of non-sulfated chondroitin, two orders of magnitude less yet an appreciable amount of heparan sulfate, and no hyaluronate were found in Caenorhabditis elegans nematodes. The chondroitin chains were heterogeneous in size, being shorter than that of whale cartilage chondroitin sulfate. The disaccharide composition analysis of heparan sulfate revealed diverse sulfation including glucosamine 2-N-sulfation, glucosamine 6-O-sulfation and uronate 2-O-sulfation. These results imply that chondroitin and heparan sulfate are involved in fundamental biological processes.

【 授权许可】

Unknown