Endoglin is expressed in the chicken vasculature and is involved in angiogenesis

FEBS Letters

Lastres, Pedro³  Cabañas, Carlos²  Raab, Ulla³  Bernabéu, Carmelo³  López-Novoa, Jose M.¹  de la Rosa, Enrique J.³  Arévalo, Miguel A.¹ 

[1]Facultad de Medicina, Universidad de Salamanca, 37007 Salamanca, Spain

[2]Facultad de Medicina, Universidad Complutense, 28040 Madrid, Spain

[3]Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Cientı́ficas (CSIC), Velazquez 144, 28006 Madrid, Spain

关键词: Endoglin;    Angiogenesis;    Chicken;    Vasculature;    Transforming growth factor β;    CAM;    chick chorioallantoic membrane;    DAB;    3;    3′-diaminobenzidine;    HHT;    hereditary hemorrhagic telangiectasia;    mAb;    monoclonal antibody;    pAb;    polyclonal antibody;    PAGE;    polyacrylamide gel electrophoresis;    TGF-β;    transforming growth factor β;   

DOI  :  10.1016/S0014-5793(99)01252-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Endoglin is a component of the transforming growth factor β (TGF-β) receptor complex, highly expressed by endothelial cells. Mutations in the endoglin gene are responsible for hereditary hemorrhagic telangiectasia type 1 (HHT1), an autosomal dominant vascular disorder caused by a haploinsufficiency mechanism. Vascular lesions (telangiectasia and arteriovenous malformations) in HHT1 are associated with loss of the capillary network, suggesting the involvement of endoglin in vascular repair processes. Using the chick chorioallantoic membrane (CAM) as an angiogenic model, we have analyzed the expression and function of chicken endoglin. A pan-specific polyclonal antibody (pAb) recognized chicken endoglin as demonstrated by immunostaining and Western blot analysis. In ovo treatment of chicken embryos with this pAb resulted in a significantly increased area of CAM. This effect was likely mediated by modulation of the ligand binding to endoglin as this pAb was able to inhibit TGF-β1 binding. These results support the involvement of endoglin in the angiogenic process.

【 授权许可】

Unknown