Isolation and purification of a 316 Da preformed compound from strawberry (Fragaria ananassa) leaves active against plant pathogens

FEBS Letters

Diaz Ricci, J¹  Farı́as, R.N¹  Castagnaro, A¹  Mamanı́ de Marchese, A¹  Filippone, M.P¹ 

[1] Departamento Bioquı́mica de la Nutrición, Instituto Superior de Investigaciones Biológicas (CONICET-UNT) and Instituto de Quı́mica Biológica ‘Dr. Bernabé Bloj’, Chacabuco 461, (4000) San Miguel de Tucumán, Tucumán, Argentina

关键词: Plant antibiotic;    Fragarin;    Phytoanticipin;    Plant defense;    Strawberry;   

DOI  :  10.1016/S0014-5793(99)01231-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An antibiotic called fragarin showing activities against bacterial and fungal plant pathogens was isolated and purified by FPLC chromatography from the soluble fraction of strawberry leaves. The molecular weight value determined by mass spectrometry is 316 Da. Fragarin remains fully active after protease treatment or alkaline hydrolysis at 100°C for 20 min. Biological and chemical analyses suggest that fragarin may be a new type of an antimicrobial preformed compound – phytoanticipin – and would constitute a primary non-specific barrier of strawberry defense.

【 授权许可】

Unknown