Molecular cloning of EDG‐3 and N‐Shc genes from the puffer fish, Fugu rubripes, and conservation of synteny with the human genome

FEBS Letters

Tokuda, Masaaki¹  Brenner, Sydney²  Yamaguchi, Fuminori²  Yamaguchi, Kumiko² 

[1] Department of 1st Physiology, Kagawa Medical University, 1750-1, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan;The Molecular Sciences Institute, 2168 Shattuck Avenue, Berkeley, CA 94704, USA

关键词: Human;    EDG-3;    N-Shc;    Chromosome mapping;    Fugu rubripes;    EDG;    endothelial differentiation protein;    GPCR;    G protein-coupled receptor;    N-Shc;    neuronal Shc;    LPA;    lysophosphatidic acid;    FISH;    fluorescence in situ hybridization;    PHA;    phytohemagglutinin;    PTB;    phosphotyrosine binding;    SH2;    Src homology 2;    CH;    collagen homology;    S1P;    sphingosine-1-phosphate;    DAPI;    4′6′-diamidino-2-phenylindole dihydrochloride;    UTR;    untranslated region;   

DOI  :  10.1016/S0014-5793(99)01210-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

EDG-3 is a receptor for sphingosine-1-phosphate mapped on human chromosome 9q22.1–q22.2. We used the compact Fugu genome for its linkage analysis. The Fugu EDG-3 was composed of one intron and two exons, encoding a 384 amino acid protein that has 56.9% homology with the human EDG-3. Approximately 3 kb apart, a neuronal Shc (N-Shc) gene was identified. It spans 7 kb containing 12 coding exons, and has an overall 53.4% similarity with the human protein. We mapped the human N-Shc gene to chromosome 9q21.3–q22.2. This is the first report of the genomic structure and the linkage of these two genes conserved between Fugu and human.

【 授权许可】

Unknown