Hepatoprotective action of adenovirus‐transferred HNF‐3γ gene in acute liver injury caused by CCl4

FEBS Letters

Sato, Kenzo²  Akiyoshi, Hideo¹  Moriyama, Masatsugu²  Shiota, Goshi³  Nakamura, Kyoko²  Nakamura, Takafumi²  Isono, Masato² 

[1] Second Department of Pathology, Faculty of Medicine, Tottori University, Yonago 683-8503, Japan;Department of Molecular Biology, Faculty of Medicine, Tottori University, Yonago 683-8503, Japan;Second Department of Internal Medicine, Faculty of Medicine, Tottori University, Yonago 683-8503, Japan

关键词: Acute liver injury;    Adenovirus;    Transcription factor;    Hepatocyte nuclear factor-3γ;    Carbon tetrachloride;    Gene therapy;    Hepatocyte growth factor;   

DOI  :  10.1016/S0014-5793(99)01202-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hepatocyte nuclear factor-3γ (HNF-3γ) is an important regulator of liver-specific genes and the expression of this factor is reduced in the liver injured by carbon tetrachloride (CCl₄). Wistar rats were infected with a recombinant adenovirus carrying the cDNA for HNF-3γ (AxCAHNF3γ) via the tail vein and were treated with CCl₄ by intraperitoneal injection. Liver damage, such as swelling of the hepatocytes and increases in serum marker enzymes were markedly alleviated by AxCAHNF3γ infection. Interestingly, hepatocyte growth factor (HGF) was strongly induced in the AxCAHNF3γ-infected liver. Likewise, HNF-1α and HNF-1β levels were increased, but HNF-3α and HNF-3β levels were depressed in the liver. Our results suggest that the transduced HNF-3γ gene leads to a hepatoprotective effect via the induction of HGF by the combined actions of liver-enriched transcription factors.

【 授权许可】

Unknown